Coding

Part:BBa_K4769209:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


gltX coding sequence from B. subtilis 3610


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1042
    Illegal AgeI site found at 861
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 407
    Illegal SapI.rc site found at 289
    Illegal SapI.rc site found at 337
    Illegal SapI.rc site found at 874
    Illegal SapI.rc site found at 1255


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: atgggaaacgaagtacgcgt (+ desired overhangs) RV: ttagatattctttaaacgctgaattgcagt (+ desired overhangs)


References